Two-step purification procedure for recombinant human asialoerythropoietin expressed in transgenic plants
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چکیده
منابع مشابه
Rapid One-Step Separation and Purification of Recombinant Phenylalanine Dehydrogenase in Aqueous Two-Phase Systems
Background: Phenylalanine dehydrogenase (PheDH EC 1.4.1.20) is a NAD+-dependent enzyme that performs the reversible oxidative deamination of L-phenylalanine to phenylpyruvate. It plays an important role in detection and screening of phenylketonuria (PKU) diseases and production of chiral intermediates as well. The main goal of this study was to find a simple and rapid alternative method for pur...
متن کاملA single step purification for recombinant proteins
A 167 base pair DNA cassette has been constructed to facilitate the detection and purification of recombinunt proteins. This cassette, kfc, cncodcs three distinct peptide units: a phosphorylation site for (he CAMP-depcndeni protein base (PKA), called hemptidc, a factor Xa cleavage site, and a calmodulin-binding peptide. Expressed kfc fusio? proteins can be purified from bacterial lysates in one...
متن کاملExpression of recombinant human acetylcholinesterase in transgenic tomato plants.
Enzyme therapy for the prevention and treatment of organophosphate poisoning depends on the availability of large amounts of cholinesterases. Transgenic plants are being evaluated for their efficiency and cost-effectiveness as a system for the bioproduction of therapeutically valuable proteins. Here we report production of a recombinant isoform of human acetylcholinesterase in transgenic tomato...
متن کاملPurification and characterization of recombinant human bile salt-stimulated lipase expressed in milk of transgenic cloned cows
Bile salt-stimulated lipase (BSSL) is a lipolytic digestive enzyme with broad substrate specificity secreted from exocrine pancreas into the intestinal lumen in all species and from the lactating mammary gland into the milk of some species, notably humans but not cows. BSSL in breast milk facilitates digestion and absorption of milk fat and promotes growth of small for gestational age preterm i...
متن کاملrapid one-step separation and purification of recombinant phenylalanine dehydrogenase in aqueous two-phase systems
background: phenylalanine dehydrogenase (phedh ec 1.4.1.20) is a nad+-dependent enzyme that performs the reversible oxidative deamination of l-phenylalanine to phenylpyruvate. it plays an important role in detection and screening of phenylketonuria (pku) diseases and production of chiral intermediates as well. the main goal of this study was to find a simple and rapid alternative method for pur...
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ژورنال
عنوان ژورنال: International Journal of Biological Macromolecules
سال: 2015
ISSN: 0141-8130
DOI: 10.1016/j.ijbiomac.2014.10.033